Pumps Calculation

The H2O2 Pumps Calculation

I used the following for data collection:
-MyWeight Gemplus 250 (accurate to .001g)
-3 ziplock sandwich bags
-30 ml Safeway brand Nasal Spray bottle with 3% H2O2 solution
-24 ml Distilled H2O
-02 ml 35% Food Grade Hydrogen Peroxide  (FGHP) 

3% Calculation
1 ml 35% FGHP = 1.118g
0.35 x 1.116g = 0.3906g H2O2

24 ml H2O + 2 ml 35% FGHP
24g + 2.238g = 26.236g H2O/H2O2 

0.7812g H2O2 / 26.236g = 0.02977
0.02978 x 100 = 2.978% H2O2 

Bag Weight
Zeroed

I weighed each individual bag marked 1, 2, and 3 carefully folding each bag the same way and ensuring the bag was centered and balanced on the scale.
Bag 1

Bag 2

Bag 3

I opened the top end of the ziplock bag proceeded to pump one full spray at roughly a 45 degree angle. This angle would more than likely be the same as one would use to inhale. The bottle's nozzle was put partially into the bag, collecting each spray in it's entirety.

Bag 1 will weigh ten individual pumps one at a time
Bag 2 will weigh five consecutive pumps
Bag 3 will weigh ten consecutive pumps

Bag 1: 01 Pump

Bag 1: 02 Pumps

Bag 1: 03 Pumps

Bag 1: 04 Pumps

Bag 1: 05 Pumps
Bag 1: 06 Pumps

Bag 1: 07 Pumps

Bag 1: 08 Pumps

Bag 1: 09 Pumps

Bag 1: 10 Pumps
Bag 2: 05 Pumps

Bag 3: 10 Pumps

Bag 1 
01 pumps H2O2 = 1.871g - 1.773g = 0.098g 
02 pumps H2O2 = 1.996g - 1.871g = 0.125g
03 pumps H2O2 = 2.066g - 1.996g = 0.070g
04 pumps H2O2 = 2.159g - 2.066g = 0.093g
05 pumps H2O2 = 2.255g - 2.159g = 0.096g
06 pumps H2O2 = 2.351g - 2.255g = 0.096g
07 pumps H2O2 = 2.451g - 2.351g = 0.100g
08 pumps H2O2 = 2.551g - 2.451g = 0.100g
09 pumps H2O2 = 2.644g - 2.551g = 0.093g
10 pumps H2O2 = 2.742g - 2.644g = 0.098g

mean = 0.0969g
standard deviation = 0.0125g

Bag 2
05 pumps H2O2 = 2.231g - 1.778g = 0.453g
average pump = 0.453g / 5 = 0.0906g

Bag 3
10 pumps H2O2 = 2.779g - 1.779g = 1.0g
average pump = 1.0g / 10 = 0.100g

Comprehensive Calculation
average = 0.0969g + 0.0906g + 0.1000g = .2875g / 3 = 0.09583g
mean = 0.0958g
standard deviation = .0039g

Probability Breakdown
68% pump is between 0.0919g and 0.0997g
95% pump is between 0.0880g and 0.1036g
It would be unusual to have a pump beyond the 95% range.

H202 Calculation
0.02978 (%) x 0.0958g (mean) = 0.00285g H2O2 per pump

01 pumps = ( 01*0.00285 ) = 0.00285g H2O2
02 pumps = ( 02*0.00285 ) = 0.00570g H2O2
03 pumps = ( 03*0.00285 ) = 0.00855g H2O2
04 pumps = ( 04*0.00285 ) = 0.01140g H2O2
05 pumps = ( 05*0.00285 ) = 0.01425g H2O2
06 pumps = ( 06*0.00285 ) = 0.01710g H2O2
07 pumps = ( 07*0.00285 ) = 0.01995g H2O2
08 pumps = ( 08*0.00285 ) = 0.02280g H2O2
09 pumps = ( 09*0.00285 ) = 0.02565g H2O2
10 pumps = ( 10*0.00285 ) = 0.02850g H2O2

Compared to Drops
01 drop 35% FGHP = 0.35 (%) x 0.04468 (mean) = 0.01564g H2O2

From personal experience, it seems that about 20-30%% of each pump does not make it into the inhale but rather hits the inside of the mouth. Therefore, 
01 pump with 20% loss = ( 0.8*0.00285g ) = 0.00228g 
01 pump with 30% loss = ( 0.7*0.00285g ) = 0.00200g 

01 drop / 01 pump
0.01564g / 0.00228g = 6.86
0.01564g / 0.00200g = 7.82

It would take approximately 6.86 - 7.82 pumps of 3% H2O2 to equal 1 ingested standard dropper drop 35% H202.

These data are an attempt to lay groundwork for something that I am not yet sure of exactly. Perhaps standardizing protocols based on factors such as ailment, body weight (Kg), and etc etc. Perhaps to correlate the data with future/past experimentations and observations.

William S. Cook